source 15q Search Results


ion exchange chromatography  (Cytiva Europe)
96
Cytiva Europe ion exchange chromatography
Ion Exchange Chromatography, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ion exchange chromatography/product/Cytiva Europe
Average 96 stars, based on 1 article reviews
ion exchange chromatography - by Bioz Stars, 2026-06
96/100 stars
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recombinant profilin  (GE Healthcare)
93
GE Healthcare recombinant profilin
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Recombinant Profilin, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/recombinant profilin/product/GE Healthcare
Average 93 stars, based on 1 article reviews
recombinant profilin - by Bioz Stars, 2026-06
93/100 stars
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anion exchange chromatography  (Danaher Inc)
96
Danaher Inc anion exchange chromatography
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Anion Exchange Chromatography, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anion exchange chromatography/product/Danaher Inc
Average 96 stars, based on 1 article reviews
anion exchange chromatography - by Bioz Stars, 2026-06
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4 6 100 pe  (Cytiva Europe)
93
Cytiva Europe 4 6 100 pe
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
4 6 100 Pe, supplied by Cytiva Europe, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/4 6 100 pe/product/Cytiva Europe
Average 93 stars, based on 1 article reviews
4 6 100 pe - by Bioz Stars, 2026-06
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source15 iso 10 100 column  (GE Healthcare)
92
GE Healthcare source15 iso 10 100 column
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Source15 Iso 10 100 Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 92 stars, based on 1 article reviews
source15 iso 10 100 column - by Bioz Stars, 2026-06
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source tm 15q hr16 10 column  (GE Healthcare)
88
GE Healthcare source tm 15q hr16 10 column
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Source Tm 15q Hr16 10 Column, supplied by GE Healthcare, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/source tm 15q hr16 10 column/product/GE Healthcare
Average 88 stars, based on 1 article reviews
source tm 15q hr16 10 column - by Bioz Stars, 2026-06
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10-ml source 15 q column  (IOtech Inc)
90
IOtech Inc 10-ml source 15 q column
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
10 Ml Source 15 Q Column, supplied by IOtech Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/10-ml source 15 q column/product/IOtech Inc
Average 90 stars, based on 1 article reviews
10-ml source 15 q column - by Bioz Stars, 2026-06
90/100 stars
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1-ml source 15q column  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc 1-ml source 15q column
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
1 Ml Source 15q Column, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/1-ml source 15q column/product/Pharmacia LKB Biotechnology Inc
Average 90 stars, based on 1 article reviews
1-ml source 15q column - by Bioz Stars, 2026-06
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source 15q  (Pharmacia LKB Biotechnology Inc)
90
Pharmacia LKB Biotechnology Inc source 15q
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Source 15q, supplied by Pharmacia LKB Biotechnology Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/source 15q/product/Pharmacia LKB Biotechnology Inc
Average 90 stars, based on 1 article reviews
source 15q - by Bioz Stars, 2026-06
90/100 stars
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source 15q column  (PerSeptive Biosystems Inc)
90
PerSeptive Biosystems Inc source 15q column
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Source 15q Column, supplied by PerSeptive Biosystems Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/source 15q column/product/PerSeptive Biosystems Inc
Average 90 stars, based on 1 article reviews
source 15q column - by Bioz Stars, 2026-06
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source 15q column  (Pfizer Inc)
86
Pfizer Inc source 15q column
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Source 15q Column, supplied by Pfizer Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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source 15q column - by Bioz Stars, 2026-06
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source 15q anionexchange column  (Danaher Inc)
86
Danaher Inc source 15q anionexchange column
Immunoblot with ( A <t>)</t> <t>recombinant</t> L. albus <t>profilin</t> and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .
Source 15q Anionexchange Column, supplied by Danaher Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/source 15q anionexchange column/product/Danaher Inc
Average 86 stars, based on 1 article reviews
source 15q anionexchange column - by Bioz Stars, 2026-06
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Image Search Results


Immunoblot with ( A ) recombinant L. albus profilin and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .

Journal: Nutrients

Article Title: Identification and Purification of Novel Low-Molecular-Weight Lupine Allergens as Components for Personalized Diagnostics

doi: 10.3390/nu13020409

Figure Lengend Snippet: Immunoblot with ( A ) recombinant L. albus profilin and ( B ) with natural L. albus extract. ( A ) (1) Tris (buffer negative control) (2) negative serum; (3) peanut allergy, lupine-sensitized (P 11); (4) peanut allergy, lupine-sensitized (P 12); (5) suspected lupine allergy (P 14); (6) peanut allergy, lupine-sensitized (P 13); (7) peanut and lupine allergy (P 15); (8) peanut allergy, lupine-sensitized (P 16); (9) peanut allergy, lupine-sensitized (P 17). ( B ) The immunoblot was designed as an inhibition assay using natural L. albus extract and sera pre-incubated with different concentrations of recombinant L. albus profilin using one profilin-reactive serum (4). (1) Tris, (2) negative serum, (3) negative serum + 50 µg rProfilin, (4) serum 4 (P 12), (5) serum 4 + 5 µg rProfilin, (6) serum 4 + 50 µg rProfilin, (7) serum 4 + 500 µg lupine extract. Blocking was performed with Synblock; serum dilution was 1:20, dilution of the HRP-conjugated mouse-anti-human IgE Fc-antibody was 1:5000 (SouthernBiotech, Birmingham, AL, USA). Immunologically, the recombinant profilin performed similarly to the natural allergen. P-code refers to .

Article Snippet: Since the purification of the recombinant profilin via further chromatography steps (size exclusion chromatography and ion-exchange chromatography (source 15Q 4.6/100 PE, GE Healthcare, Uppsala, Sweden) were not successful, it was further purified via preparative SDS-PAGE.

Techniques: Western Blot, Recombinant, Negative Control, Inhibition, Incubation, Blocking Assay